Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Novel domains of expression for orphan receptor tyrosine kinase Ror2 in the human and mouse reproductive system

doi: 10.1002/dvdy.24138

Figure Lengend Snippet: Expression of Ror2 in the developing mouse gonad and ducts. A–F′ Ror2 expression was assessed using whole mount ISH. A′ is a hand cut slice and C′,D′,E′,F′ are 100μm vibratome sections through the mesonephros (yellow dotted lines) following whole mount ISH. The gonads are demarcated from the mesonephros by a black dotted line in all panels. At E11.5, Ror2 expression was detected most highly in the developing mesonephros (below the outlined gonad in A) and the Wolffian duct (A′). Ror2 sense control probe was used on male gonads at E13.5 and no expression was detected (B). At E12.5 expression is seen in the gonad, mesonephros, Wolffian ducts and Müllerian ducts in both the female (C,C′) and male (D,D′). At E13.5 similar expression is seen in the female and male gonads and in the ducts throughout the middle of the mesonephros (E,E′,F,F′). Black dotted line: gonad; blue arrows: Wolffian duct; pink arrows: Müllerian duct.

Article Snippet: These two parallel structures persist in the mesonephros until E15.5, after which Müllerian ducts regress in males in response to anti Müllerian hormone (also known as Müllerian inhibitory substance) secreted by the Sertoli cells of the testis ( Behringer et al., 1994 ; Mishina et al., 1996 ; Belville et al., 1999 ; Hoshiya et al., 2003 ).

Techniques: Expressing, Control

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Novel domains of expression for orphan receptor tyrosine kinase Ror2 in the human and mouse reproductive system

doi: 10.1002/dvdy.24138

Figure Lengend Snippet: Expression of Ror2 in the epithelium of developing ducts. A,B IF on cryosections of P0 and P21 male epididymis. Inset in A shows Ror2 expression in the epithelium of the epididymal ducts. C,D IF on cryosections of adult female ductal structures derived from the Müllerian duct. Similar to the male, Ror2 expression (red) is seen in the epithelium of the ducts colocalizing with E-Cad expression (C,D, grey). Inset in C shows membrane epithelial staining in the ductal derivative. E IF on cryosections of gestational day 11 pregnant female uterus. E′ Magnified region marked with yellow box in E. Expression is seen in the epithelium of the uterus (E,E′). Expression is higher on the basal side (yellow arrow) of the epithelium as compared to the luminal side. Expression is also seen in the glands (white arrow) beneath the epithelium. F IF on cryosections of human adult uterus. Similar to the mouse, ROR2 expression is higher on the basal side (yellow arrow) of the epithelium as compared to the luminal side. Scale bar in A,B,C,D,E,F,G is 50μm, and F′ is 10μm.

Article Snippet: These two parallel structures persist in the mesonephros until E15.5, after which Müllerian ducts regress in males in response to anti Müllerian hormone (also known as Müllerian inhibitory substance) secreted by the Sertoli cells of the testis ( Behringer et al., 1994 ; Mishina et al., 1996 ; Belville et al., 1999 ; Hoshiya et al., 2003 ).

Techniques: Expressing, Derivative Assay, Membrane, Staining